Thiol activated cytolysin
WebDec 1, 2001 · LLO, a pore-forming toxin of the thiol-activated cholesterol-dependent cytolysins (CDCs) family, is secreted as a soluble monomer that oligomerizes upon … WebMar 18, 2002 · Several members of the pore-forming CDCs family, formerly called thiol-activated toxins, have been characterized in detail. They include streptolysin O (SLO) of Streptococcus pyogenes and perfringolysin O (PFO) of Clostridium perfringens.
Thiol activated cytolysin
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WebJan 15, 2000 · The thiol-activated cytolysin (TACY) family is a prominent group of bacterial toxins, of which streptolysin O (SLO) is the prototype. Members of this group are elaborated by more than 20 species of Gram-positive bacteria [1] ( Table 1 ) and are intimately involved in the pathogenesis of infections by species such as Arcanobacterium pyogenes ... The term "Cytolysin" or "Cytolytic toxin" was first introduced by Alan Bernheimer to describe membrane damaging toxins (MDTs) that have cytolytic effects to cells. The first kind of cytolytic toxin discovered have hemolytic effects on erythrocytes of certain sensitive species, such as Human. For this reason "Hemolysin" was first used to describe any MDTs. In the 1960s certain MDTs were proved to be destructive on cells other than erythrocytes, such as leukocytes. The ter…
WebJan 12, 2001 · Structure of a cholesterol-binding, thiol-activated cytolysin and a model of its membrane form. Cell. 1997; 89: 685-692. Abstract; Full Text; Full Text PDF; PubMed; Scopus (402) Google Scholar) that may contain up to 50 individual monomers. The resulting transmembrane pore can be up to 30 nm in diameter (38. Sekiya K ; WebDec 1, 2016 · Botulinolysin (BLY) is a toxin produced by Clostridium botulinum that belongs to a group of thiol-activated hemolysins. In this study, a protein exhibiting hemolytic activity was purified from the culture supernatant of C. botulinum serotype D strain 4947. The purified protein displayed a single band by sodium dodecyl sulfate polyacrylamide gel …
WebJan 26, 2013 · The toxin has a thiol-activated cytolysin which causes lysis of human platelets, lysosomes and a variety of subcellular membranes. The existing therapy seems to have challenged as available vaccines are not so effective and the bacteria developed resistance to many drugs. Computer aided approach is a novel platform to screen drug … Web1.C.12 The Thiol-activated Cholesterol-dependent Cytolysin (CDC) Family. Cholesterol-binding 'sulfhydryl-activated' toxins bind to cholesterol containing animal cell membranes and can be reversibly inactivated by oxidation. The prototype of the family, perfringolysin O (PFO), can lyse cholesterol-containing membranes of eukaryotic host cells.
WebThiol-Activated Cytolysin and a Model of Its Membrane Form Jamie Rossjohn,* Susanne C. Feil,* family is referred to as thiol-activated cytolysins be-William J. McKinstry,* Rodney K. Tweten,† cause biochemical modification of a cysteine residue and Michael W. Parker* causes toxin inactivation, although this residue is not
WebA distinctive feature of the thiol-activated toxins is the formation of extremely large arc- and ring-shaped structures in target membranes. These structures are believed to represent … flowers dnepropetrovskWebDec 31, 2024 · Among the toxins produced by serotype C and D strains, botulinolysin (BLY), a thiol-activated hemolysin, is a member of the CDC family. In contrast to BoNT, which has been intensively studied because of its extraordinarily potent toxicity, the pathogenic role of BLY remains unclear. flowers dmsWebpfam01289 (PSSM ID: 426182): Conserved Protein Domain Family Thiol_cytolysin, flowers-doWebNov 1, 2011 · Streptolysin O, a 63 kDa exotoxin coded by slo gene, is a well-characterised thiol-activated cytolysin, which damages cholesterol-containing membranes resulting in disruption and lysis of the... flowers diy easyWebMay 30, 1997 · The mechanisms by which proteins gain entry into membranes is a fundamental problem in biology. Here, we present the first crystal structure of a thiol-activated cytolysin, perfringolysin O, a member of a large family of toxins that kill eukaryotic cells by punching holes in their membranes. The molecule adopts an unusually elongated … green aviation awardWebMay 30, 1997 · Here, we present the first crystal structure of a thiol-activated cytolysin, perfringolysin O, a member of a large family of toxins that kill eukaryotic cells by punching … green aventurine metaphysical meaningThe thiol-activated Cholesterol-dependent Cytolysin (CDC) family (TC# 1.C.12) is a member of the MACPF superfamily. Cholesterol dependent cytolysins are a family of β-barrel pore-forming exotoxins that are secreted by gram-positive bacteria. CDCs are secreted as water-soluble monomers of 50-70 kDa, that when bound to the target cell, form a circular homo-oligomeric complex containing as many as 40 (or more) monomers. Through multiple conformational chan… green avenue shamshabad