WebWe used a fluorescence conjugated transferrin (FITC-Tf) to monitor the trafficking of TfR1. Cells were incubated with 20 μg/ml FITC-Tf on ice for 20 min. At low temperatures, Tf still bound to TfR1 on the cell surface, but endocytosis was terminated. After the unbound FITC-Tf was washed away, the cells were transferred to 37 C to reinitiate ... WebFITC transferrin is a green fluorescent transferrin conjugate with excitation/emission wavelength at 490 nm/515 nm. Nanocs also provides a number of orange, red, purple, blue and IR dye labeled transferrin products with excitation/emission wavelength. These labeled transferrin products were purified by size chromatography to remove non ...

Tumor cell-imposed iron restriction drives immunosuppressive ...

WebFeb 16, 2005 · FITC-transferrin (3 μg/ml) and Cy5-transferrin (0.5 μg/ml) were then bound to HeLa cells (1 h, 4°C), and cells were subsequently warmed to 37°C in the absence or … WebAlexa Fluor® 488. Alexa Fluor® 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are … east texas neurology lufkin tx

Transferrin Uptake Assays Transferrin Recycling using …

WebHuman transferrin is encoded by the TF gene and is an iron-binding blood plasma glycoprotein that controls the level of free iron in biological fluids. Human transferrin … WebFlow Cytometry - FITC Anti-Transferrin Receptor antibody [MEM-75] (ab239251) Flow cytometric analysis of K562 cells (human chronic myelogenous leukemia cell line from … WebRat monoclonal Transferrin Receptor antibody [YTA 74.4] conjugated to FITC. Validated in Flow Cyt and tested in Mouse. Cited in 4 publication(s). Immunogen corresponding to tissue, cells or virus. cumberland town hall maine