Ephexin5

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August undefined, 2024

WebMoreover, lowering neuronal Ephexin5 levels inhibited new spine outgrowth in response to both global increases in neural activity and local glutamatergic stimulation of the dendrite, suggesting that Ephexin5 is necessary for activity-dependent spine outgrowth. Our data support a model in which Ephexin5 serves a dual role in spinogenesis, acting ... WebJan 24, 2024 · Ephexin5 has an important role in angiogenesis because it mediates the VEGF-induced Rho GTPase activity modulation. During angiogenesis, Ephexin5 is highly expressed in retinal endothelial cells where Ephexin5 promotes actin polymerization by regulating the activity of Cdc42 and RhoJ [34,78]. By increasing Cdc42 activity and …

Ephecting Excitatory Synapse Development - ScienceDirect

WebSep 30, 2024 · Ephexin5 is a putative UBE3A substrate that has restricted expression early in development, regulates synapse formation during hippocampal development, and is … WebFeb 27, 2024 · This work was funded by the National Institutes of Health (R01 MH102364-02 awarded to SS Margolis.), the NSF Graduate Research Fellowship (grant no. 1232825 awarded to GL Sell), the Department of Neuroscience graduate program at Johns Hopkins University School of Medicine (5T32EY017203-15 from 09/1/2012 to 8/31/2013 awarded … copper window flashing

A dual role for the RhoGEF Ephexin5 in regulation of dendritic …

WebIn contrast, Ephexin5 A/A-myc, which failed to activate RhoA, also failed to suppress spine growth (Figure 7C). To test if Ephexin5 serine residues 107 and 109 contributed to dendritic outgrowth, we performed a Sholl analysis to measure dendritic complexity in neurons previously transfected with Ephexin5-myc or Ephexin5 A/A-myc. WebOct 29, 2010 · Ephexin5 suppresses this activity, and EphB2 relieves this repression by phosphorylating and directing Ephexin5 for degradation by the E3 ligase Ube3A . These findings cement EphBs as a key regulator of excitatory synapse development and suggest the interesting possibility that other known synaptogenic factors will have similarly … WebFeb 7, 2024 · Ephexin5-GFP is enriched at sites of new spine outgrowth (A) Images of dendrites from neurons (DIV 9-10) co-transfected at DIV 7-8 with E5-GFP + DsRedExpress (DsRed) and treated with bicuculline to enhance the rate of new spine outgrowth. Blue arrows indicate sites of new spine outgrowth. Time stamps are relative to time of new … famous native american social workers

Seth Margolis - The Solomon H Snyder Department of Neuroscience

Protein That Regulates Brain Cell Connections Could Be …

WebMar 21, 2024 · EXPH5 (Exophilin 5) is a Protein Coding gene. Diseases associated with EXPH5 include Epidermolysis Bullosa Simplex 4, Localized Or Generalized Intermediate, … WebHere, we examined the role of the RhoGEF Ephexin5 in driving activity-dependent spine outgrowth. We found that reducing Ephexin5 levels increased spine outgrowth, and … copper winding wire price per kgWebApr 1, 2024 · Ephexin5 is not the sole target of the proteasome in regulating spine outgrowth. We previously proposed a model of activity-dependent spine outgrowth in which neural activity led to local proteasome activation that then caused the degradation of inhibitory factors blocking spine outgrowth (Hamilton et al., 2012). If E5 were the sole … copper windmills for your yard

"WebMar 27, 2024 · "Ephexin5 is a tantalizing pharmaceutical target because in otherwise healthy adults, there's very little present in the brain," says Gabrielle L. Sell, a graduate student at the Johns Hopkins ... " - Ephexin5

Ephexin5

EXPH5 exophilin 5 - NIH Genetic Testing Registry (GTR) - NCBI

• Son of sevenless (SOS1) is an important GEF in the cell growth-regulatory MAPK/ERK pathway. SOS1 binds GRB2 at the plasma membrane after EGF receptor activation. SOS1 activates the small G protein Ras. • eIF-2b is a eukaryotic initiation factor necessary to initiate protein translation. eIF-2b regenerates the GTP-bound form of eIF-2 for an additional cycle in protein synthesis initiation, i.e., its binding to the Met-t-RNA. WebOct 29, 2010 · Ephexin5 GEF Domain Analysis, Related to Figure 2 (A) Lysates from 293 cells previously transfected with empty vector (Control), E5-Myc, or E1-Myc were assayed for activated Rac1 and Cdc42 using the PBD pull-down assay. Pull-down lanes were immunoblotted with mouse anti-Rac1 and reprobed with rabbit anti-Cdc42 (top two …

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WebEphexin5 is a RhoA guanine nucleotide exchange factor (RhoAGEF). Molecularly, Ephexin5 is known to exchange GTP (guanine triphosphate) for GDP (guanine … Webannexin 5. A member of a highly conserved protein family that bind acidic phospholipids in a calcium-dependent manner and help flag cells destined for apoptotic death by …

WebEphexin5 guanine nucleotide exchange activity we compared its Dbl-homology (DH) domain to the DH domain of other RhoA-speciﬁc GEFs (Snyder et al., 2002). We identiﬁed within the a5 helix of E5’s DH domain three amino acids that are conserved in other GEFs that, like E5, activate RhoA but not Rac1 and Cdc42 (Figure S2B). To generate a form ... WebApr 1, 2024 · Notably, Ephexin5 is ubiquitinated and degraded by the ubiquitin proteasome system (UPS) in response to stimulation of the EphB2 receptor tyrosine kinase (Margolis et al., 2010). Furthermore, Ephexin5 acts as a negative regulator of spine and synapse density in neuronal cultures (Margolis et al., 2010); however, whether Ephexin5 acts to inhibit ...

WebMoreover, we identify a substrate of PKCε, Ephexin5, whose early-elevated expression in developing neurons may in part explain the mechanism by which PKCε plays seemingly opposing roles that depend on neuronal maturity. Schaffer et al. define roles in developing hippocampal neurons for specific PKCs, linking neuronal PKCε activation to ... WebNov 27, 2024 · Ephexin5 wild-type and Ephexin5 null genetic backgrounds were performed in neurons cultured from mouse neonates of C57BL/6 or 129 lineage dissected at P0-P1, with tail snippets saved for ...

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WebFeb 1, 2024 · Ephexin5 is a GEF that activates RhoA in neurons [122]. Intriguingly, their results also showed that the expression of Ephexin5 is increased at sites in dendrites where future new spines are ... famous native american shamansWebThese data indicate that Ephexin5 is capable of modulating disease behavioral, cell biological, and electrophysiological phenotypes. Therefore, we found Ephexin5 could be … copper window awnings exteriorWebThe 'lollipop plot' above illustrates recurrent (observed in 3 or more out of 4440 TCGA tumor samples from 15 cancer types) and therefore potentially oncogenic missense mutations … copper winding wire standardWebOct 29, 2010 · Ephexin5 Is a GEF that Activates RhoA (A) Lysates from 293 cells transfected with empty vector (Ctrl) or E5-Myc overexpressing vector (WT) were assayed … famous native american speakersWebEphexin5 guanine nucleotide exchange activity we compared its Dbl-homology (DH) domain to the DH domain of other RhoA-speciﬁc GEFs (Snyder et al., 2002). We identiﬁed within the a5 helix of E5’s DH domain three amino acids that are conserved in other GEFs that, like E5, activate RhoA but not Rac1 and Cdc42 (Figure S2B). To generate a form ... copper windsor panWebThe emergence of Ephexin5 as a therapeutic target in Alzheimer's disease Expert Opin Ther Targets. 2024 Apr;23(4):263-265. doi: 10.1080/14728222.2024.1586884. Epub 2024 Mar 5. Authors Emily K Cook 1 , Gabrielle L Sell 2 , Thomas B Schaffer 3 , Seth S Margolis 1 4 Affiliations 1 a Department of Biological Chemistry , The ... famous native american storiesWebMar 27, 2024 · Ephexin5 is a GEF that activates the small G protein RhoA, a regulator of the actin cytoskeleton. Genetic loss- and gain-of-function studies indicate that Ephexin5 … famous native americans in us history